Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation
نویسندگان
چکیده
منابع مشابه
Methylated DNA immunoprecipitation.
The identification of DNA methylation patterns is a common procedure in the study of epigenetics, as methylation is known to have significant effects on gene expression, and is involved with normal development as well as disease. Thus, the ability to discriminate between methylated DNA and non-methylated DNA is essential for generating methylation profiles for such studies. Methylated DNA immun...
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DNA modifications, such as methylation and hydroxymethylation, are pivotal players in modulating gene expression, genomic imprinting, X-chromosome inactivation, and silencing repetitive sequences during embryonic development. Aberrant DNA modifications lead to embryonic and postnatal abnormalities and serious human diseases, such as cancer. Comprehensive genome-wide DNA methylation and hydroxym...
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We have developeda simple and reproducible fingerprinting method for screeningthegenomefor regionsof DNA thathavealteredpatternsof DNA methylationassociatedwith oncogenictransformation.Restriction enzymes with different sensitivities to cytosine methylation in their rec ognition sites were used to digest genomic DNAs from primary tumors, cell lines, and normal tissuesprior to arbitrarily primed...
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CpG island promoter hypermethylation of tumor suppressor genes is a common hallmark of human cancer, and new largescale epigenomic technologies might be useful in our attempts to define the complete DNA hypermethylome of tumor cells. Here, we report a functional search for hypermethylated CpG islands using the colorectal cancer cell line HCT-116, in which two major DNA methyltransferases, DNMT1...
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CpG island promoter hypermethylation of tumor suppressor genes is a common hallmark of human cancer, and new large-scale epigenomic technologies might be useful in our attempts to define the complete DNA hypermethylome of tumor cells. Here, we report a functional search for hypermethylated CpG islands using the colorectal cancer cell line HCT-116, in which two major DNA methyltransferases, DNMT...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2016
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1990